Since having fluid drained from his injured right thumb on Tuesday, Upton hadn't started, although he pinch-ran Wednesday against the Pirates late in the game.
The D-backs, currently on a four-game losing streak, haven't won since Upton last started. The club is also without outfielder Chris Young, who went on the disabled list on Wednesday.
"Justin knows what's going on around here. He wants to play," D-backs manager Kirk Gibson said. "He's got a pad in his glove to help protect it, but that's it."
Upton originally hurt the thumb April 8 but tried to battle through it for six games before taking a step back with rest and rehab.
Gibson said Upton met with doctors Friday and they informed him, barring jamming it again, he can't further injure the thumb.