"He's going to be fine," D-backs manager A.J. Hinch said. "It's the old 'day-to-day' but I'm expecting him to play tomorrow."
Upton left Wednesday's game against the White Sox with the sprain after he legged out an infield single in the second inning. He is hitting .333 in 14 games this spring.
"I'm day-to-day and we'll see how it feels," Upton said. "I felt good running through the stuff we had to do. It's getting better and I hope it gets better every day."
Upton went through all of his normal activities Thursday morning but said he "wasn't moving great." He hopes to play Friday.
"It depends how I wake up and feel in the morning," he said. "I'm going to test it out."
Upton hit .300 with 26 homers and 86 RBIs last season. He recently signed a six-year contract extension worth $51.25 million.
Jesse Sanchez is a national reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.